I re-activated all my plugins, created two new sites, checked existing sites and everything's still up and running so yes, I think so!
Perhaps I should have found that link myself - but then again, if a Bitnami installation of WP Multisite should include this step, should the the step not be included in the installer? Subdirectory-based WP Multisite is nothing exotic afaik and yet it gets no mention on this page. Might have saved us both some time.
On the other hand, I reached out to the community and got a helpful reply for which I'm grateful and that counts for something