I can see these lines at the end of your
# Include "/opt/bitnami/apache2/conf/bitnami/bitnami.conf"
It looks like you have commented out the
/opt/bitnami/apache2/conf/bitnami/bitnami.conf created by us and that you are using a custom file
/opt/bitnami/apache2/conf/bitnami/httpd.conf created by you. Can you try uncommenting the
bitnami.conf file, commenting the
httpd.conf file, and restarting Apache?