I don't understand very well what you're trying to setup.
AWS lambda is a serverless platform. Do you want to integrate any function you developed there with a Mediawiki instance?
Please note that Bitnami Cloud Images can be launched from EC2, the AWS marketplace or Bitnami launchpads. What are your concerns about using EC2 to manage your instances?
To use Aurora, you can follow the guide below. By default Bitnami VMs use a MySQL/MariaDB which is included on the stack but they can be configured to use a external database such as RDS/Aurora:
Regarding S3, what is the purpose of using it? do you want to configure Mediawiki to store data there (like icons or avatars)?
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