Thanks for using Bitnami. Did you restart NGINX after updating the configuration for changes to take effect?
sudo /opt/bitnami/ctlscript.sh restart nginx
Can you also tell us which guide are you using to include those parameters? Is that required for any plugin or theme?
Apart from that, we have a Support Tool that will gather relevant information for us to analyze your configuration and logs. Could you please execute it on the machine where the stack is running by following the steps described in the guide below?
Please note that you need to paste the code ID that is shown at the end.